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pegfp vamp4 homo sapiens  (Addgene inc)
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Addgene inc pegfp vamp4 homo sapiens
Representative confocal fluorescence micrograph of HeLa cells co‐transfected for 24 h to produce mCherry‐BspF and GFP‐BspF and treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 and 2 µm (insets). Representative confocal fluorescence micrographs of HeLa cells co‐transfected for 24 h to produce mCherry‐BspF and either GFP‐MICAL‐L1, GFP‐STX16, GFP‐STX6, or GFP‐VAMP3 and treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 and 2 µm (insets). Localization of GFP‐MICAL‐L1, GFP‐STX16, GFP‐STX6, or GFP‐VAMP3 to mCherry‐BspF‐labeled tubules was quantified in at least 300 individual cells per experiment. Data are means ± SD from n = 3 independent experiments. Representative confocal fluorescence micrographs of HeLa cells co‐transfected for 24 h to produce mCherry‐BspF and either GFP‐Rab11a or <t>VAMP4‐GFP</t> and treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 and 2 µm (insets). Localization of GFP‐Rab11a or VAMP4‐GFP to mCherry‐BspF‐labeled tubules was quantified in at least 300 individual cells per experiment. Data are means ± SD from n = 3 independent experiments.
Pegfp Vamp4 Homo Sapiens, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
pegfp vamp4 homo sapiens - by Bioz Stars, 2026-03
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Representative confocal fluorescence micrograph of HeLa cells co‐transfected for 24 h to produce mCherry‐BspF and GFP‐BspF and treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 and 2 µm (insets). Representative confocal fluorescence micrographs of HeLa cells co‐transfected for 24 h to produce mCherry‐BspF and either GFP‐MICAL‐L1, GFP‐STX16, GFP‐STX6, or GFP‐VAMP3 and treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 and 2 µm (insets). Localization of GFP‐MICAL‐L1, GFP‐STX16, GFP‐STX6, or GFP‐VAMP3 to mCherry‐BspF‐labeled tubules was quantified in at least 300 individual cells per experiment. Data are means ± SD from n = 3 independent experiments. Representative confocal fluorescence micrographs of HeLa cells co‐transfected for 24 h to produce mCherry‐BspF and either GFP‐Rab11a or VAMP4‐GFP and treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 and 2 µm (insets). Localization of GFP‐Rab11a or VAMP4‐GFP to mCherry‐BspF‐labeled tubules was quantified in at least 300 individual cells per experiment. Data are means ± SD from n = 3 independent experiments.

Journal: The EMBO Journal

Article Title: A Brucella effector modulates the Arf6‐Rab8a GTPase cascade to promote intravacuolar replication

doi: 10.15252/embj.2021107664

Figure Lengend Snippet: Representative confocal fluorescence micrograph of HeLa cells co‐transfected for 24 h to produce mCherry‐BspF and GFP‐BspF and treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 and 2 µm (insets). Representative confocal fluorescence micrographs of HeLa cells co‐transfected for 24 h to produce mCherry‐BspF and either GFP‐MICAL‐L1, GFP‐STX16, GFP‐STX6, or GFP‐VAMP3 and treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 and 2 µm (insets). Localization of GFP‐MICAL‐L1, GFP‐STX16, GFP‐STX6, or GFP‐VAMP3 to mCherry‐BspF‐labeled tubules was quantified in at least 300 individual cells per experiment. Data are means ± SD from n = 3 independent experiments. Representative confocal fluorescence micrographs of HeLa cells co‐transfected for 24 h to produce mCherry‐BspF and either GFP‐Rab11a or VAMP4‐GFP and treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 and 2 µm (insets). Localization of GFP‐Rab11a or VAMP4‐GFP to mCherry‐BspF‐labeled tubules was quantified in at least 300 individual cells per experiment. Data are means ± SD from n = 3 independent experiments.

Article Snippet: pEGFP‐ VAMP4 Homo sapiens , Addgene , Cat#42313.

Techniques: Fluorescence, Transfection, Labeling